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Portrait of Heiner Linke; Photo: Kennet Ruona

Heiner Linke

Professor, Deputy dean at Faculty of Engineering, LTH

Portrait of Heiner Linke; Photo: Kennet Ruona

Construction and Characterization of Kilobasepair Densely Labeled Peptide-DNA

Author

  • Suzana Kovacic
  • Laleh Samii
  • Guillaume Lamour
  • Hongbin Li
  • Heiner Linke
  • Elizabeth H. C. Bromley
  • Derek N. Woolfson
  • Paul M. G. Curmi
  • Nancy R. Forde

Summary, in English

Directed assembly of biocompatible materials benefits from modular building blocks in which structural organization is independent of introduced functional modifications. For soft materials, such modifications have been limited. Here, long DNA is successfully functionalized with dense decoration by peptides. Following introduction of alkyne-modified nucleotides into kilobasepair DNA, measurements of persistence length show that DNA mechanics are unaltered by the dense incorporation of alkynes (similar to 1 alkyne/2 bp) and after click-chemistry attachment of a tunable density of peptides. Proteolytic cleavage of densely tethered peptides (similar to 1 peptide/3 bp) demonstrates addressability of the functional groups, showing that this accessible approach to creating hybrid structures can maintain orthogonality between backbone mechanics and overlaid function. The synthesis and characterization of these hybrid constructs establishes the groundwork for their implementation in future applications, such as building blocks in modular approaches to a range of problems in synthetic biology.

Department/s

  • Solid State Physics
  • NanoLund

Publishing year

2014

Language

English

Pages

4065-4072

Publication/Series

Biomacromolecules

Volume

15

Issue

11

Document type

Journal article

Publisher

The American Chemical Society (ACS)

Topic

  • Condensed Matter Physics

Status

Published

ISBN/ISSN/Other

  • ISSN: 1526-4602