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Portrait of Heiner Linke; Photo: Kennet Ruona

Heiner Linke

Professor, Deputy dean at Faculty of Engineering, LTH

Portrait of Heiner Linke; Photo: Kennet Ruona

Surface nanostructures for fluorescence probing of supported lipid bilayers on reflective substrates.

Author

  • Aleksandra Dabkowska
  • Gaelle Piret
  • Cassandra Niman
  • Mercy Lard
  • Heiner Linke
  • Tommy Nylander
  • Christelle Prinz

Summary, in English

The fluorescence interference contrast (FLIC) effect prevents the use of fluorescence techniques to probe the continuity and fluidity of supported lipid bilayers on reflective materials due to a lack of detectable fluorescence. Here we show that adding nanostructures onto reflective surfaces to locally confer a certain distance between the deposited fluorophores and the reflecting surface enables fluorescence detection on the nanostuctures. The nanostructures consist of either deposited nanoparticles or epitaxial nanowires directly grown on the substrate and are designed such that they can support a lipid bilayer. This simple method increases the fluorescence signal sufficiently to enable bilayer fluorescence detection and to observe the recovery of fluorescence after photobleaching in order to assess lipid bilayer formation on any reflective surface.

Department/s

  • Physical Chemistry
  • NanoLund
  • Solid State Physics
  • Neuronano Research Center (NRC)

Publishing year

2015

Language

English

Pages

18020-18024

Publication/Series

Nanoscale

Volume

7

Issue

43

Document type

Journal article

Publisher

Royal Society of Chemistry

Topic

  • Biophysics

Status

Published

Research group

  • Neuronano Research Center (NRC)

ISBN/ISSN/Other

  • ISSN: 2040-3372