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Portrait of Heiner Linke; Photo: Kennet Ruona

Heiner Linke

Professor, Deputy dean at Faculty of Engineering, LTH

Portrait of Heiner Linke; Photo: Kennet Ruona

Construction of a Chassis for a Tripartite Protein-Based Molecular Motor

Author

  • Lara S.R. Small
  • Marc Bruning
  • Andrew R. Thomson
  • Aimee L. Boyle
  • Roberta B. Davies
  • Paul M G Curmi
  • Nancy R. Forde
  • Heiner Linke
  • Derek N Woolfson
  • Elizabeth H. C. Bromley

Summary, in English

Improving our understanding of biological motors, both to fully comprehend their activities in vital processes, and to exploit their impressive abilities for use in bionanotechnology, is highly desirable. One means of understanding these systems is through the production of synthetic molecular motors. We demonstrate the use of orthogonal coiled-coil dimers (including both parallel and antiparallel coiled coils) as a hub for linking other components of a previously described synthetic molecular motor, the Tumbleweed. We use circular dichroism, analytical ultracentrifugation, dynamic light scattering, and disulfide rearrangement studies to demonstrate the ability of this six-peptide set to form the structure designed for the Tumbleweed motor. The successful formation of a suitable hub structure is both a test of the transferability of design rules for protein folding as well as an important step in the production of a synthetic protein-based molecular motor.

Department/s

  • NanoLund
  • Solid State Physics

Publishing year

2017-06-16

Language

English

Pages

1096-1102

Publication/Series

ACS Synthetic Biology

Volume

6

Issue

6

Document type

Journal article

Publisher

The American Chemical Society (ACS)

Topic

  • Medical Materials
  • Nano Technology

Status

Published

ISBN/ISSN/Other

  • ISSN: 2161-5063