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Portrait of Heiner Linke; Photo: Kennet Ruona

Heiner Linke

Professor, Deputy dean at Faculty of Engineering, LTH

Portrait of Heiner Linke; Photo: Kennet Ruona

Synthetic biology approaches to dissecting linear motor protein function : towards the design and synthesis of artificial autonomous protein walkers

Author

  • Heiner Linke
  • Birte Höcker
  • Ken’ya Furuta
  • Nancy R. Forde
  • Paul M.G. Curmi

Summary, in English

Molecular motors and machines are essential for all cellular processes that together enable life. Built from proteins with a wide range of properties, functionalities and performance characteristics, biological motors perform complex tasks and can transduce chemical energy into mechanical work more efficiently than human-made combustion engines. Sophisticated studies of biological protein motors have provided many structural and biophysical insights and enabled the development of models for motor function. However, from the study of highly evolved, biological motors, it remains difficult to discern detailed mechanisms, for example, about the relative role of different force generation mechanisms, or how information is communicated across a protein to achieve the necessary coordination. A promising, complementary approach to answering these questions is to build synthetic protein motors from the bottom up. Indeed, much effort has been invested in functional protein design, but so far, the “holy grail” of designing and building a functional synthetic protein motor has not been realized. Here, we review the progress made to date, and we put forward a roadmap for achieving the aim of constructing the first artificial, autonomously running protein motor. Specifically, we propose to break down the task into (i) enzymatic control of track binding, (ii) the engineering of asymmetry and (iii) the engineering of allosteric control for internal communication. We also propose specific approaches for solving each of these challenges.

Department/s

  • NanoLund
  • Solid State Physics

Publishing year

2020-08

Language

English

Pages

1041-1054

Publication/Series

Biophysical Reviews

Volume

12

Issue

4

Document type

Journal article review

Publisher

Springer

Topic

  • Biophysics

Keywords

  • Allostery
  • Energy transduction
  • Motor protein
  • Processivity
  • Synthetic biology
  • Thermal fluctuations

Status

Published

ISBN/ISSN/Other

  • ISSN: 1867-2450