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Portrait of Ivan Maximov. Photo: Kennet Ruona

Ivan Maximov

Associate Professor, Coordinator Exploratory Nanotechnology

Portrait of Ivan Maximov. Photo: Kennet Ruona

A Method for Investigation of Size-Dependent Protein Binding to Nanoholes Using Intrinsic Fluorescence of Proteins

Author

  • Bita Malekian
  • Ivan Maximov
  • Rainer Timm
  • Tommy Cedervall
  • Dan Hessman

Summary, in English

We have developed a novel method to study the influence of surface nanotopography on human fibrinogen adsorption at a given surface chemistry. Well-ordered arrays of nanoholes with different diameters down to 45 nm and a depth of 50 nm were fabricated in silicon by electron beam lithography and reactive ion etching. The nanostructured chip was used as a model system to understand the effect of size of the nanoholes on fibrinogen adsorption. Fluorescence imaging, using the intrinsic fluorescence of proteins, was used to characterize the effect of the nanoholes on fibrinogen adsorption. Atomic force microscopy was used as a complementary technique for further characterization of the interaction. The results demonstrate that as the size of the nanoholes is reduced to 45 nm, fibrinogen adsorption is significantly increased.

Department/s

  • Solid State Physics
  • Synchrotron Radiation Research
  • Biochemistry and Structural Biology
  • NanoLund

Publishing year

2017-08-31

Language

English

Pages

4772-4778

Publication/Series

ACS Omega

Volume

2

Issue

8

Document type

Journal article

Publisher

The American Chemical Society (ACS)

Topic

  • Subatomic Physics

Status

Published

ISBN/ISSN/Other

  • ISSN: 2470-1343