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Portrait of Jonas Tegenfeldt. Photo: Kennet Ruona

Jonas Tegenfeldt

Professor, Coordinator Nanobiology & Neuronanoscience

Portrait of Jonas Tegenfeldt. Photo: Kennet Ruona

From immobilized cells to motile cells on a bed-of-nails: effects of vertical nanowire array density on cell behaviour.

Author

  • Henrik Persson
  • Zhen Li
  • Jonas O Tegenfeldt
  • Stina Oredsson
  • Christelle Prinz

Summary, in English

The field of vertical nanowire array-based applications in cell biology is growing rapidly and an increasing number of applications are being explored. These applications almost invariably rely on the physical properties of the nanowire arrays, creating a need for a better understanding of how their physical properties affect cell behaviour. Here, we investigate the effects of nanowire density on cell migration, division and morphology for murine fibroblasts. Our results show that few nanowires are sufficient to immobilize cells, while a high nanowire spatial density enables a "bed-of-nails" regime, where cells reside on top of the nanowires and are fully motile. The presence of nanowires decreases the cell proliferation rate, even in the "bed-of-nails" regime. We show that the cell morphology strongly depends on the nanowire density. Cells cultured on low (0.1 μm(-2)) and medium (1 μm(-2)) density substrates exhibit an increased number of multi-nucleated cells and micronuclei. These were not observed in cells cultured on high nanowire density substrates (4 μm(-2)). The results offer important guidelines to minimize cell-function perturbations on nanowire arrays. Moreover, these findings offer the possibility to tune cell proliferation and migration independently by adjusting the nanowire density, which may have applications in drug testing.

Department/s

  • Solid State Physics
  • NanoLund
  • BioCARE: Biomarkers in Cancer Medicine improving Health Care, Education and Innovation
  • Functional zoology
  • Neuronano Research Center (NRC)

Publishing year

2015

Language

English

Publication/Series

Scientific Reports

Volume

5

Document type

Journal article

Publisher

Nature Publishing Group

Topic

  • Medical Biotechnology

Status

Published

Research group

  • Neuronano Research Center (NRC)

ISBN/ISSN/Other

  • ISSN: 2045-2322