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Portrait of Jonas Tegenfeldt. Photo: Kennet Ruona

Jonas Tegenfeldt

Professor, Coordinator Nanobiology & Neuronanoscience

Portrait of Jonas Tegenfeldt. Photo: Kennet Ruona

Long DNA isolation and imaging using lateral displacement arrays integrated with DNA combing

Author

  • Oskar E. Ström
  • Jason P. Beech
  • Jonas O. Tegenfeldt

Summary, in English

We demonstrate proof of principle of an efficient and simple method combining sample preparation and stretching of DNA in a single device that can be used as basis for an integrated portable system for optical DNA mapping. An important bottleneck for DNA mapping, as well as for other methods, is the isolation of long DNA molecules from a background of contaminants such as shorter DNA fragments, dyes, and lysate. We demonstrate the isolation of longer (48.5 kbp or 166 kbp) from shorter (<10 kbp) DNA strands using Deterministic Lateral Displacement (DLD), followed by surface-stretching of the sorted DNA for mapping.

Department/s

  • Solid State Physics
  • NanoLund

Publishing year

2019

Language

English

Pages

771-772

Publication/Series

23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019

Document type

Conference paper

Publisher

Chemical and Biological Microsystems Society

Topic

  • Biochemistry and Molecular Biology
  • Condensed Matter Physics

Keywords

  • DLD
  • DNA sample preparation
  • DNA Separation
  • DNA stretching
  • Molecular Combing

Conference name

23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019

Conference date

2019-10-27 - 2019-10-31

Conference place

Basel, Switzerland

Status

Published

ISBN/ISSN/Other

  • ISBN: 9781733419000