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Portrait of Jonas Tegenfeldt. Photo: Kennet Ruona

Jonas Tegenfeldt

Professor, Coordinator Nanobiology & Neuronanoscience

Portrait of Jonas Tegenfeldt. Photo: Kennet Ruona

Microfluidic separation of parasites and parasite-infected cells from blood for the diagnosis of leishmaniasis

Author

  • C. Regnault
  • K. Punyani
  • S. Holm
  • J. P. Beech
  • Marcia C. de Oliveira Otto
  • Kevan C Herold
  • M. Herbig
  • J. Guck
  • J. O. Tegenfeldt
  • Michael P Barrett

Summary, in English

Microfluidic techniques were applied to the separation of parasite and parasite-infected cells from blood to facilitate the detection of leishmaniasis, a disease representing a high burden in the developing world and for which new diagnostic tools are urgently needed. Leishmania mexicana promastigotes were successfully separated from red blood cells in a deterministic lateral displacement device. The mechanical properties of macrophages infected with L. mexicana were investigated using real-time deformability cytometry. In the early stage, we find that macrophages deform less than the control. The trend is reversed four days post infection while we see a continuous increase in cell size after parasitization.

Department/s

  • Solid State Physics
  • NanoLund

Publishing year

2016

Language

English

Pages

244-245

Publication/Series

20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

Document type

Conference paper

Publisher

Chemical and Biological Microsystems Society

Topic

  • Medical Laboratory and Measurements Technologies

Keywords

  • Deformability
  • Diagnosis
  • Leishmaniasis
  • Sorting

Conference name

20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

Conference date

2016-10-09 - 2016-10-13

Conference place

Dublin, Ireland

Status

Published

ISBN/ISSN/Other

  • ISBN: 9780979806490