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Portrait of Jonas Tegenfeldt. Photo: Kennet Ruona

Jonas Tegenfeldt

Professor, Coordinator Nanobiology & Neuronanoscience

Portrait of Jonas Tegenfeldt. Photo: Kennet Ruona

Fluorescence enhancement from single DNA molecules confined in SiO 2 nanochannels

Author

  • F. Westerlund
  • F. Persson
  • A. Kristensen
  • J. O. Tegenfeldt

Summary, in English

We demonstrate that the detected emission intensity from YOYO-labeled DNA molecules confined in 180 nm deep Si/SiO2 nanofunnels changes significantly and not monotonically with the width of the funnel, an emission enhancement that is only detected for emitted light polarized parallel to the channel. We explain the enhancement effect as being due to optical phenomena in the channels. The enhancement effect may be of importance for quantitative fluorescence microscopy and for experiments with a tight photon budget.

Department/s

  • Department of Physics
  • NanoLund
  • Solid State Physics

Publishing year

2010-12-01

Language

English

Pages

578-580

Publication/Series

14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010

Volume

1

Document type

Conference paper

Topic

  • Atom and Molecular Physics and Optics

Keywords

  • DNA
  • Fluorescence microscopy
  • Nanofluidics
  • Single molecule

Conference name

14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010

Conference date

2010-10-03 - 2010-10-07

Conference place

Groningen, Netherlands

Status

Published

ISBN/ISSN/Other

  • ISBN: 9781618390622