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Portrait of Jonas Tegenfeldt. Photo: Kennet Ruona

Jonas Tegenfeldt

Professor, Coordinator Nanobiology & Neuronanoscience

Portrait of Jonas Tegenfeldt. Photo: Kennet Ruona

Improving the instrumental resolution of sensors based on localized surface plasmon resonance

Author

  • Andreas Dahlin
  • Jonas Tegenfeldt
  • Fredrik Höök

Summary, in English

The colorimetric variations induced upon changes in interfacial refractive index of nanoscale noble metal structures exhibiting localized surface plasmon resonance (LSPR) provides a convenient means of label-free, affinity-based detection of biomolecular recognition reactions. However, despite being similar in nature to conventional SPR, LSPR has so far suffered from significantly lower data quality in terms of its signal-to-noise ratio (S/N) in typical biomolecular recognition analysis. In this work, generic data analysis algorithms and a simple experimental setup that provide a S/N upon protein binding that is comparable to that of state-of-the art SPR systems are presented. Specifically, it is demonstrated how temporal variations ( rate similar to 0.5 Hz) in parameters proportional to the resonance peak position can be recorded simultaneously, yielding a peak position precision of < 5 x 10(-4) nm and an extinction noise level of < 5 x 10(-6) absorbance units (Abs). This, in turn, is shown to provide a S/N of similar to 2000 ( equivalent to a detection limit of < 0.1 ng/cm(2)) for typical protein binding reactions. Furthermore, the importance of utilizing changes in both peak position and magnitude is highlighted by comparing different LSPR active noble metal architectures that respond differently to bulk and interfacial refractive index changes.

Department/s

  • Solid State Physics

Publishing year

2006

Language

English

Pages

4416-4423

Publication/Series

Analytical Chemistry

Volume

78

Issue

13

Document type

Journal article

Publisher

The American Chemical Society (ACS)

Topic

  • Analytical Chemistry

Status

Published

ISBN/ISSN/Other

  • ISSN: 1520-6882