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Portrait of Jonas Tegenfeldt. Photo: Kennet Ruona

Jonas Tegenfeldt

Professor, Coordinator Nanobiology & Neuronanoscience

Portrait of Jonas Tegenfeldt. Photo: Kennet Ruona

DNA sample cleanup using deterministic lateral displacement

Author

  • Rebekah Kim
  • Alexandra Kühnlein
  • Darren A. Smith
  • Bao Dang Ho
  • Assaf Grunwald
  • Jason P. Beech
  • Robert K. Neely
  • Yuval Ebenstein
  • Jonas O. Tegenfeldt

Summary, in English

Optical mapping relies on the preparation of fluorescent DNA. DNA must be imaged with good signal to noise and therefore the background of unwanted DNA fragments, fluorescent dyes and other reagents need to be removed. We use deterministic lateral displacement to separate 48.5 kbp DNA from < 10 kbp DNA. We also show the removal of 48.5 kbp DNA fragments from a background of fluorescent ATTO647N molecules and the recovery of >50 kbp molecules from a background of shorter digested fragments. In both cases improving signal to noise during imaging.

Department/s

  • Solid State Physics
  • BioCARE: Biomarkers in Cancer Medicine improving Health Care, Education and Innovation
  • NanoLund

Publishing year

2016

Language

English

Pages

1527-1528

Publication/Series

20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

Document type

Conference paper

Publisher

Chemical and Biological Microsystems Society

Topic

  • Medical Laboratory and Measurements Technologies

Keywords

  • Deterministic lateral displacement
  • DNA
  • Sample cleanup
  • Separation

Conference name

20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

Conference date

2016-10-09 - 2016-10-13

Conference place

Dublin, Ireland

Status

Published

ISBN/ISSN/Other

  • ISBN: 9780979806490