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Portrait of Jonas Tegenfeldt. Photo: Kennet Ruona

Jonas Tegenfeldt

Professor, Coordinator Nanobiology & Neuronanoscience

Portrait of Jonas Tegenfeldt. Photo: Kennet Ruona

Applications of optical DNA mapping in microbiology

Author

  • Diana Bogas
  • Lena Nyberg
  • Rui Pacheco
  • Nuno F. Azevedo
  • Jason P. Beech
  • Margarita Gomila
  • Jorge Lalucat
  • Célia M. Manaia
  • Olga C. Nunes
  • Jonas O. Tegenfeldt
  • Fredrik Westerlund

Summary, in English

Optical mapping (OM) has been used in microbiology for the past 20 years, initially as a technique to facilitate DNA sequence-based studies; however, with decreases in DNA sequencing costs and increases in sequence output from automated sequencing platforms, OM has grown into an important auxiliary tool for genome assembly and comparison. Currently, there are a number of new and exciting applications for OM in the field of microbiology, including investigation of disease outbreaks, identification of specific genes of clinical and/or epidemiological relevance, and the possibility of single-cell analysis when combined with cell-sorting approaches. In addition, designing lab-on-a-chip systems based on OM is now feasible and will allow the integrated and automated microbiological analysis of biological fluids. Here, we review the basic technology of OM, detail the current state of the art of the field, and look ahead to possible future developments in OM technology for microbiological applications.

Department/s

  • Solid State Physics
  • NanoLund

Publishing year

2017

Language

English

Pages

255-267

Publication/Series

BioTechniques

Volume

62

Issue

6

Document type

Journal article review

Publisher

Informa Healthcare

Topic

  • Microbiology
  • Medical Laboratory and Measurements Technologies

Keywords

  • DNA-based identification
  • Imaging technique
  • Lab-on-a chip
  • Microbiological clinical diagnosis
  • Optical DNA mapping

Status

Published

ISBN/ISSN/Other

  • ISSN: 0736-6205