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Portrait of Jonas Tegenfeldt. Photo: Kennet Ruona

Jonas Tegenfeldt

Professor, Coordinator Nanobiology & Neuronanoscience

Portrait of Jonas Tegenfeldt. Photo: Kennet Ruona

Lipid-Based Passivation in Nanofluidics

Author

  • Fredrik Persson
  • Joachim Fritzsche
  • Kalim U. Mir
  • Mauro Modesti
  • Fredrik Westerlund
  • Jonas Tegenfeldt

Summary, in English

Stretching DNA in nanochannels is a useful tool for direct, visual studies of genomic DNA at the single molecule level. To facilitate the study of the interaction of linear DNA with proteins in nanochannels, we have implemented a highly effective passivation scheme based on lipid bilayers. We demonstrate virtually complete long-term passivation of nanochannel surfaces to a range of relevant reagents, including streptavidin-coated quantum dots, RecA proteins, and RecA-DNA complexes. We show that the performance of the lipid bilayer is significantly better than that of standard bovine serum albumin-based passivation. Finally, we show how the passivated devices allow us to monitor single DNA cleavage events during enzymatic degradation by DNase I. We expect that our approach will open up for detailed, systematic studies of a wide range of protein-DNA interactions with high spatial and temporal resolution.

Department/s

  • Solid State Physics
  • NanoLund

Publishing year

2012

Language

English

Pages

2260-2265

Publication/Series

Nano Letters

Volume

12

Issue

5

Document type

Journal article

Publisher

The American Chemical Society (ACS)

Topic

  • Nano Technology

Keywords

  • Nanofluidics
  • passivation
  • antifouling
  • lipid bilayer
  • protein-DNA
  • interactions
  • single molecules

Status

Published

ISBN/ISSN/Other

  • ISSN: 1530-6992