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Portrait of Jonas Tegenfeldt. Photo: Kennet Ruona

Jonas Tegenfeldt

Professor, Coordinator Nanobiology & Neuronanoscience

Portrait of Jonas Tegenfeldt. Photo: Kennet Ruona

Separation of 100-kilobase DNA molecules in 10 seconds

Author

  • O. Bakajin
  • T. A J Duke
  • J. Tegenfeldt
  • C. F. Chou
  • S. S. Chan
  • R. H. Austin
  • E. C. Cox

Summary, in English

Long double-stranded DNA molecules were separated in microfabricated hexagonal arrays in less than 1 min, several orders of magnitude faster than by using conventional technology. DNA samples were first concentrated at the entrance to the array in a thin band by entropic focusing. They were then separated by pulsed field electrophoresis. T4 (168.9 kbp) and λ (48.5 kbp) DNAs could be resolved into two clearly separated bands in ∼10 s in these experiments. This corresponds to a mass resolution of 6% in 11 min in a 1-cm-long array.

Department/s

  • Solid State Physics
  • Department of Physics

Publishing year

2001-12-15

Language

English

Pages

6053-6056

Publication/Series

Analytical Chemistry

Volume

73

Issue

24

Document type

Journal article

Publisher

The American Chemical Society (ACS)

Status

Published

ISBN/ISSN/Other

  • ISSN: 0003-2700