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Portrait of Jonas Tegenfeldt. Photo: Kennet Ruona

Jonas Tegenfeldt

Professor, Coordinator Nanobiology & Neuronanoscience

Portrait of Jonas Tegenfeldt. Photo: Kennet Ruona

Integrated on-chip system for DNA purification, labelling and surface stretching

Author

  • Oskar E. Ström
  • Jason P. Beech
  • Jonas O. Tegenfeldt

Summary, in English

We report a simplified, integrated and more efficient way of preparing DNA for analysis. We have developed a microfluidic platform that can purify DNA and subsequently label and stretch it on a surface. We have successfully extracted DNA from a gel-filled microfluidic channel using electrophoresis and subsequently stretched the DNA linearly by absorption to either a hydrophobic or a positively-charged surface. To our knowledge, DNA has not previously been stretched on to a surface while being moved using an electric field in this way. We have also labelled DNA after stretching with intercalating dyes. The intention, following stretching and labelling, is to analyze the DNA with optical mapping.

Department/s

  • NanoLund

Publishing year

2018

Language

English

Pages

1177-1180

Publication/Series

22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018

Volume

2

Document type

Conference paper

Publisher

Chemical and Biological Microsystems Society

Topic

  • Physical Chemistry

Keywords

  • DNA labelling
  • DNA Purification
  • Molecular Combing

Conference name

22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018

Conference date

2018-11-11 - 2018-11-15

Conference place

Kaohsiung, Taiwan, Province of China

Status

Published

ISBN/ISSN/Other

  • ISBN: 9781510897571