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Portrait of Jonas Tegenfeldt. Photo: Kennet Ruona

Jonas Tegenfeldt

Professor, Coordinator Nanobiology & Neuronanoscience

Portrait of Jonas Tegenfeldt. Photo: Kennet Ruona

What do photons do to fluorescently stained DNA in confinement?

Author

  • J. P. Beech
  • Lena Nyberg
  • Joachim Fritzsche
  • Fredrik Westerlund
  • J. O. Tegenfeldt

Summary, in English

We have studied a selection of factors influencing the damage of DNA in nanochannels during fluorescence imaging. For cutting and nicking of DNA we show that the DNA is shortened during imaging. To avoid photodamage over the course of several hours of a typical experiment, we demonstrate the importance of an oxygen free gas to propel the buffer solution through the device. Finally, by varying the size of the channels, we show indications that higher DNA concentrations lead to higher rates of photodamage necessitating a balance between needs for highly stretched DNA and needs for long measurement times.

Department/s

  • Solid State Physics
  • NanoLund

Publishing year

2013-01-01

Language

English

Pages

5-7

Publication/Series

17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013

Volume

1

Document type

Conference paper

Publisher

Chemical and Biological Microsystems Society

Topic

  • Biophysics

Keywords

  • Confinement
  • DNA
  • Intercalating dye
  • Nano-channels
  • Photobleaching

Conference name

17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013

Conference date

2013-10-27 - 2013-10-31

Conference place

Freiburg, Germany

Status

Published

ISBN/ISSN/Other

  • ISBN: 9781632666246