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Portrait of Jonas Tegenfeldt. Photo: Kennet Ruona

Jonas Tegenfeldt

Professor, Coordinator Nanobiology & Neuronanoscience

Portrait of Jonas Tegenfeldt. Photo: Kennet Ruona

Fluorescence microscopy of nanochannel-confined DNA

Author

  • Fredrik Persson
  • Fredrik Westerlund
  • Jonas O. Tegenfeldt

Editor

  • Erwin J.G. Peterman
  • Gijs J.L. Wuite

Summary, in English

Stretching of DNA in nanoscale confinement allows for direct visualization of the genetic contents of the DNA on the single DNA molecule level. DNA stretched in nanoscale confinement also allows for studies of DNA-protein interactions and DNA polymer physics in confined environments. This chapter describes the basic steps to fabricate the nanostructures, to perform the experiments, and to analyze the data.

Department/s

  • Department of Physics
  • NanoLund
  • Solid State Physics

Publishing year

2011-10-24

Language

English

Pages

159-179

Publication/Series

Methods in Molecular Biology

Volume

783

Document type

Book chapter

Topic

  • Biophysics

Keywords

  • DNA
  • Fluorescence
  • Nanochannels
  • Single molecule

Status

Published

ISBN/ISSN/Other

  • ISSN: 1064-3745
  • ISBN: 9781617792816