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Portrait of Jonas Tegenfeldt. Photo: Kennet Ruona

Jonas Tegenfeldt

Professor, Coordinator Nanobiology & Neuronanoscience

Portrait of Jonas Tegenfeldt. Photo: Kennet Ruona

DNA concentration wave formation in pillar arrays

Author

  • Oskar E. Ström
  • Jason P. Beech
  • Jonas O. Tegenfeldt

Summary, in English

High throughput in particle and molecular sorting schemes is an important performance indicator and can be realized through increased volumetric processing rates or increased concentrations. Here we investigate the effect of increased concentration of high-molecular weight DNA in micropillar arrays for deterministic lateral displacement (DLD). We find that the array imposes regular fluctuations in the concentration of the DNA if the sample concentration and flow rates exceed respective threshold values. We characterize the resulting concentration waves and study their influence on microsphere trajectories in the device. We expect our results to be relevant e.g. for sample preparation of cell lysates which can often be complicated by the release of chromosomal DNA.

Department/s

  • Solid State Physics
  • NanoLund
  • LUCC - Lund University Cancer Centre

Publishing year

2020

Language

English

Pages

240-241

Publication/Series

MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Document type

Conference paper

Publisher

Chemical and Biological Microsystems Society

Topic

  • Biochemistry and Molecular Biology
  • Condensed Matter Physics

Keywords

  • DNA concentration waves
  • DNA entanglement
  • Pillar Arrays

Conference name

24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020

Conference date

2020-10-04 - 2020-10-09

Conference place

Virtual, Online

Status

Published

ISBN/ISSN/Other

  • ISBN: 9781733419017