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Portrait of Jonas Tegenfeldt. Photo: Kennet Ruona

Jonas Tegenfeldt

Professor, Coordinator Nanobiology & Neuronanoscience

Portrait of Jonas Tegenfeldt. Photo: Kennet Ruona

The dynamics of genomic-length DNA molecules in 100-nm channels

Author

  • Jonas Tegenfeldt
  • Christelle Prinz
  • H Cao
  • S Chou
  • WW Reisner
  • R Riehn
  • YM Wang
  • EC Cox
  • JC Sturm
  • P Silberzan
  • RH Austin

Summary, in English

We show that genomic-length DNA molecules imaged in nano-channels have an extension along the channel that scales linearly with the contour length of the polymer, in agreement with the scaling arguments developed by de Gennes for self-avoiding confined polymers. This fundamental relationship allows us to measure directly the contour length of single DNA molecules confined in the channels, and the statistical analysis of the dynamics of the polymer in the nanochannel allows us to compute the SD of the mean of the extension. This statistical analysis allows us to measure the extension of A DNA multimers with a 130-nm SD in 1 min.

Department/s

  • Solid State Physics

Publishing year

2004

Language

English

Pages

10979-10983

Publication/Series

Proceedings of the National Academy of Sciences

Volume

101

Issue

30

Document type

Journal article

Publisher

National Acad Sciences

Topic

  • Condensed Matter Physics

Status

Published

ISBN/ISSN/Other

  • ISSN: 1091-6490