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Portrait of Jonas Tegenfeldt. Photo: Kennet Ruona

Jonas Tegenfeldt

Professor, Coordinator Nanobiology & Neuronanoscience

Portrait of Jonas Tegenfeldt. Photo: Kennet Ruona

DNA in nanochannels-directly visualizing genomic information

Author

  • Fredrik Persson
  • Jonas Tegenfeldt

Summary, in English

The power of nanofluidic channels to analyze DNA is described along with practical experimental hints. As an introduction, a general overview is given on conventional DNA analysis tools, as well as tools under development towards the $1000 genome. The focus of this tutorial review is the stretching of DNA in nanoscale channels for coarse-grained mapping of DNA. To understand the behavior of the DNA, basic theory is discussed. Experimental details are revealed so that the reader, with the proper equipment, should be able to perform experiments. Basic approaches to the analysis of the data are discussed. Finally, potential future directions are discussed including the application of melting mapping as a simple barcode for the DNA.

Department/s

  • Solid State Physics
  • NanoLund

Publishing year

2010

Language

English

Pages

985-999

Publication/Series

Chemical Society Reviews

Volume

39

Issue

3

Document type

Journal article review

Publisher

Royal Society of Chemistry

Topic

  • Condensed Matter Physics

Status

Published

ISBN/ISSN/Other

  • ISSN: 0306-0012