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Portrait of Jonas Tegenfeldt. Photo: Kennet Ruona

Jonas Tegenfeldt

Professor, Coordinator Nanobiology & Neuronanoscience

Portrait of Jonas Tegenfeldt. Photo: Kennet Ruona

Sample preparation for single-cell whole chromosome analysis

Author

  • Jason P. Beech
  • Karl Adolfsson
  • Stefan Holm
  • Farnaz Yadegari
  • Camilla Freitag
  • Joachim Fritzsche
  • Kalim U. Mir
  • Jonas O. Tegenfeldt

Summary, in English

In this work we present an integrated system for whole chromosome analysis of single bacterium. Using whole genome barcoding techniques, which offer direct and rapid microscopic visualization of the entire genome in one field-of-view, we aim to rapidly identify individual bacterium. We are developing our device to achieve the crucial, and difficult process of isolating a bacterium, removing the DNA in one piece and transferring it to a nano-channel for visualisation. In order to achieve control over the bacteria we encapsulate them in agarose, using flow focusing. The encapsulated bacteria can then be transported in microchannels to proximity with the nanochannels and then chemically lysis can be performed. Following lysis the intact genome can be extracted and transferred to the meandering nanochannel for analysis. We believe this device holds the potential to significantly decrease analysis times for single cell, whole genome analysis with the potential of opening up for automated, high-throughput genome analysis in microfluidic systems.

Department/s

  • Solid State Physics
  • NanoLund

Publishing year

2012-01-01

Language

English

Pages

998-999

Publication/Series

Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

Document type

Conference paper

Publisher

Chemical and Biological Microsystems Society

Topic

  • Other Medical Biotechnology

Keywords

  • Bacteria encapsulation
  • Chromosome analysis
  • Melting mapping
  • Nanochannels

Conference name

16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

Conference date

2012-10-28 - 2012-11-01

Conference place

Okinawa, Japan

Status

Published

ISBN/ISSN/Other

  • ISBN: 9780979806452