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Portrait of Jonas Tegenfeldt. Photo: Kennet Ruona

Jonas Tegenfeldt

Professor, Coordinator Nanobiology & Neuronanoscience

Portrait of Jonas Tegenfeldt. Photo: Kennet Ruona

Fluorescence microscopy of nanochannel-confined DNA

Author

  • Fredrik Westerlund
  • Fredrik Persson
  • Joachim Fritzsche
  • Jason P. Beech
  • Jonas O. Tegenfeldt

Summary, in English

Stretching of DNA in nanoscale confinement allows for several important studies. The genetic contents of the DNA can be visualized on the single DNA molecule level and both the polymer physics of confined DNA and also DNA/protein and other DNA/DNA-binding molecule interactions can be explored. This chapter describes the basic steps to fabricate the nanostructures, perform the experiments and analyze the data.

Department/s

  • Solid State Physics
  • NanoLund

Publishing year

2018

Language

English

Pages

173-198

Publication/Series

Methods in Molecular Biology

Volume

1665

Document type

Book chapter

Publisher

Humana Press

Topic

  • Nano Technology
  • Biochemistry and Molecular Biology

Keywords

  • DNA
  • Fluorescence
  • Nanochannels
  • Single molecule

Status

Published

ISBN/ISSN/Other

  • ISSN: 10643745