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Portrait of Jonas Tegenfeldt. Photo: Kennet Ruona

Jonas Tegenfeldt

Professor, Coordinator Nanobiology & Neuronanoscience

Portrait of Jonas Tegenfeldt. Photo: Kennet Ruona

A lipid-based passivation scheme for nanofluidics

Author

  • Joachim Fritzsche
  • Fredrik Persson
  • Kalim U. Mir
  • Mauro Modesti
  • Fredrik Westerlund
  • Jonas O. Tegenfeldt

Summary, in English

Stretching DNA in nanochannels allows for direct, visual studies of genomic DNA at the single molecule level. In order to facilitate the study of the interaction of linear DNA with proteins in nanochannels, we have implemented a highly effective passivation scheme based on lipid bilayers. We show long-term passivation of nanochannel surfaces to several relevant reagents and demonstrate that the performance of the lipid bilayer is significantly better compared to standard bovine serum albumin-based passivation. Moreover, we demonstrate how the passivated devices allow us to monitor single DNA cleavage events during enzymatic degradation.

Department/s

  • NanoLund
  • Solid State Physics

Publishing year

2012-01-01

Language

English

Pages

1876-1878

Publication/Series

Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

Document type

Conference paper

Publisher

Chemical and Biological Microsystems Society

Topic

  • Biophysics

Keywords

  • Lipid bilayer
  • Nanofluidics
  • Passivation
  • Protein-DNA interactions
  • Single molecules

Conference name

16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

Conference date

2012-10-28 - 2012-11-01

Conference place

Okinawa, Japan

Status

Published

ISBN/ISSN/Other

  • ISBN: 9780979806452