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Portrait of Jonas Tegenfeldt. Photo: Kennet Ruona

Jonas Tegenfeldt

Professor, Coordinator Nanobiology & Neuronanoscience

Portrait of Jonas Tegenfeldt. Photo: Kennet Ruona

Presence of vitronectin and activated complement factor C9 on ventriculoperitoneal shunts and temporary ventricular drainage catheters

Author

  • Fredrik Lundberg
  • Dai Qing Li
  • Dan Falkenback
  • Tor Lea
  • Peter Siesjö
  • Sven Söderström
  • Bohdan J. Kudryk
  • Jonas O. Tegenfeldt
  • Sadahiro Nomura
  • Åsa Ljungh

Summary, in English

Object. The pathogenesis of cerebrospinal fluid (CSF) shunt infection is characterized by staphylococcal adhesion to the polymeric surface of the shunt catheter. Proteins from the CSF-fibronectin, vitronectin, and fibrinogen-are adsorbed to the surface of the catheter immediately after insertion. These proteins can interfere with the biological systems of the host and mediate staphylococcal adhesion to the surface of the catheter. In the present study, the presence of fibronectin, vitronectin, and fibrinogen on CSF shunts and temporary ventricular drainage catheters is shown. The presence of fragments of fibrinogen is also examined. Methods. The authors used the following methods: binding radiolabeled antibodies to the catheter surface, immunoblotting of catheter eluates, and scanning force microscopy of immunogold bound to the catheter surface. The immunoblot showed that vitronectin was adsorbed in its native form and that fibronectin was degraded into small fragments. Furthermore, the study demonstrated that the level of vitronectin in CSF increased in patients with an impaired CSF-blood barrier. To study complement activation, an antibody that recognizes the neoepitope of activated complement factor C9 was used. The presence of activated complement factor C9 was shown on both temporary catheters and shunts. Conclusions. Activation of complement close to the surface of an inserted catheter could contribute to the pathogenesis of CSF shunt infection.

Department/s

  • Division of Medical Microbiology
  • Neurosurgery
  • Solid State Physics

Publishing year

1999-01-01

Language

English

Pages

101-108

Publication/Series

Journal of Neurosurgery

Volume

90

Issue

1

Document type

Journal article

Publisher

American Association of Neurosurgeons

Topic

  • Microbiology in the medical area
  • Other Basic Medicine

Keywords

  • Activated complement C9
  • Fibrinogen
  • Immunogold
  • Scanning force microscopy
  • Ventricular catheter
  • Vitronectin

Status

Published

ISBN/ISSN/Other

  • ISSN: 0022-3085