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Knut Deppert

Knut Deppert

Professor

Knut Deppert

Direct deposition of gas phase generated aerosol gold nanoparticles into biological fluids - corona formation and particle size shifts.

Author

  • Christian Svensson
  • Maria Messing
  • Martin Lundqvist
  • Alexander Schollin
  • Knut Deppert
  • Joakim Pagels
  • Jenny Rissler
  • Tommy Cedervall

Summary, in English

An ongoing discussion whether traditional toxicological methods are sufficient to evaluate the risks associated with nanoparticle inhalation has led to the emergence of Air-Liquid interface toxicology. As a step in this process, this study explores the evolution of particle characteristics as they move from the airborne state into physiological solution. Airborne gold nanoparticles (AuNP) are generated using an evaporation-condensation technique. Spherical and agglomerate AuNPs are deposited into physiological solutions of increasing biological complexity. The AuNP size is characterized in air as mobility diameter and in liquid as hydrodynamic diameter. AuNP:Protein aggregation in physiological solutions is determined using dynamic light scattering, particle tracking analysis, and UV absorption spectroscopy. AuNPs deposited into homocysteine buffer form large gold-aggregates. Spherical AuNPs deposited in solutions of albumin were trapped at the Air-Liquid interface but was readily suspended in the solutions with a size close to that of the airborne particles, indicating that AuNP:Protein complex formation is promoted. Deposition into serum and lung fluid resulted in larger complexes, reflecting the formation of a more complex protein corona. UV absorption spectroscopy indicated no further aggregation of the AuNPs after deposition in solution. The corona of the deposited AuNPs shows differences compared to AuNPs generated in suspension. Deposition of AuNPs from the aerosol phase into biological fluids offers a method to study the protein corona formed, upon inhalation and deposition in the lungs in a more realistic way compared to particle liquid suspensions. This is important since the protein corona together with key particle properties (e.g. size, shape and surface reactivity) to a large extent may determine the nanoparticle effects and possible translocation to other organs.

Department/s

  • NanoLund
  • Ergonomics and Aerosol Technology
  • Solid State Physics
  • Synchrotron Radiation Research
  • Biochemistry and Structural Biology
  • MERGE - ModElling the Regional and Global Earth system

Publishing year

2013

Language

English

Publication/Series

PLoS ONE

Volume

8

Issue

9

Document type

Journal article

Publisher

Public Library of Science

Topic

  • Mechanical Engineering
  • Biological Sciences
  • Physical Sciences

Status

Published

ISBN/ISSN/Other

  • ISSN: 1932-6203