Maria Thereza Perez
Limitation of Anatomical Integration between Subretinal Transplants and the Host Retina.
Summary, in English
Methods. Sprague-Dawley (SD) rat retinal tissue from embryonic day (E)18 was subretinally grafted to adult (60-day-old) normal SD rats, to RCS rats (32 and 73 days old), and to adult (60-day-old) transgenic P23H rats. After various survival times (28–183 days), transplanted retinas were processed for routine histology and immunocytochemistry. Antibodies against calbindin, neuronal nitric oxide synthase (NOS), and protein kinase C (PKC) were used to identify specific retinal cell types and their processes.
Results. The shape and position of the immunoreactive cell bodies indicated that the expected neuronal populations were labeled within the grafts and in the host retina. Labeled neuronal processes were also observed. In each case, NOS-, calbindin-, and PKC-immunolabeled fibers formed bridges between the graft and the host tissues. However, regardless of the extent of host photoreceptor cell loss, the age of the recipient, or the genetic background, bridging fibers were observed only in areas where the host photoreceptor layer was discontinuous or completely missing.
Conclusions. The present study demonstrates that the host photoreceptor layer plays a role in limiting graft–host anatomical integration.
- Ophthalmology, Lund
Investigative Ophthalmology & Visual Science
Association for Research in Vision and Ophthalmology Inc.
- ISSN: 1552-5783