Monica Kåredal
Researcher
Mass spectrometric monitoring of in vitro formed ad ducts between hexahydrophthalic anhydride (HHPA) and cysteine and lysine
Author
Summary, in English
The in vitro formed adducts between hexahydrophthalic anhydride (HHPA) and cysteine and lysine were monitored by mass spectrometry. Freshly synthesized N-acetyl-S-hexahydrophthaloyl-L-cysteine was dissolved in dry acetonitrile and added to a solution of N-acetyl-L-lysine in 0.1 M sodium phosphate buffer (pH 7.4) an incubated at 37°C. The reactions were monitored by LC/MS on a triple quadrupole mass spectrometer with electrospray ionization coupled to a HPLC system from Perkin Elmer. The results show that there was a rapid rearrangement of HHPA from most of the cysteines to the lysines, and that HHPA-adducts of cysteine will be transferred to lysine when encountered.
Department/s
- Division of Occupational and Environmental Medicine, Lund University
- Dermatology and Venereology (Lund)
Publishing year
2002
Language
English
Pages
325-326
Publication/Series
Proceedings 50th ASMS Conference on Mass Spectrmetry and Allied Topics
Document type
Conference paper
Publisher
American Society for Mass Spectrometry, Santa Fe, NM, 87505, United States
Topic
- Environmental Health and Occupational Health
Keywords
- Electrospray ionization (ESI)
- Multiple reaction monitoring (MRM)
- Hexahydrophthalic anhydride (HHPA)
- Ion spray voltage
Conference name
Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Conference date
2002-06-02 - 2002-06-06
Conference place
Orlando, FL, United States
Status
Published