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Portrait of Sara Snogerup Linse

Sara Linse

Professor

Portrait of Sara Snogerup Linse

Solubility of Aβ40 peptide

Author

  • Veronica Lattanzi
  • Katja Bernfur
  • Emma Sparr
  • Ulf Olsson
  • Sara Linse

Summary, in English

In this work we measured, by using a direct approach, the equilibrium solubility of recombinant Aβ40 peptide to be S ​= ​0.36 ​± ​0.15 ​μM in aqueous solution of 20 ​mM sodium phosphate buffer at pH 7.4. Microfluidic diffusional sizing (MDS) and mass spectrometry (MALDI-TOF/TOF) with isotope standard were used to quantify the concentration of soluble Aβ40 species coexisting with formed fibrils. A sample set of Aβ40 at different concentrations was incubated at 37 ​°C under quiescent conditions and for different incubation times. After incubation, the samples were centrifuged and the concentration of Aβ40 in the supernatant was determined. This allowed us to also determine the metastable zone in supersaturated Aβ40 monomer solutions, as a function of the observation time, as well as the monomer concentration coexisting with formed fibrils. Moreover, the hydrodynamic radius (RH) of the Aβ40 species detected in the supernatant at different time points was also measured, and showed a constant value of ∼1.8 ​nm, consistent with random coil Aβ40 monomers.

Department/s

  • Biochemistry and Structural Biology
  • Physical Chemistry
  • MultiPark: Multidisciplinary research focused on Parkinson´s disease
  • NanoLund: Center for Nanoscience

Publishing year

2021

Language

English

Publication/Series

JCIS Open

Volume

4

Document type

Journal article

Topic

  • Biochemistry and Molecular Biology

Keywords

  • Alzheimer
  • Mass spectrometry
  • Metastable regime
  • Microfluidics diffusion sizing
  • Recombinant amyloid beta 40
  • Solubility

Status

Published