The browser you are using is not supported by this website. All versions of Internet Explorer are no longer supported, either by us or Microsoft (read more here:

Please use a modern browser to fully experience our website, such as the newest versions of Edge, Chrome, Firefox or Safari etc.

Portrait of Tommy Cedervall; Photo: Kennet Ruona

Tommy Cedervall

Associate Professor, Coordinator Nanosafety

Portrait of Tommy Cedervall; Photo: Kennet Ruona

Biocompatibility of mannan nanogel-safe interaction with plasma proteins


  • Silvia A. Ferreira
  • Cecilia Oslakovic
  • Risto Cukalevski
  • Birgitta Frohm
  • Björn Dahlbäck
  • Sara Linse
  • Francisco M. Gama
  • Tommy Cedervall

Summary, in English

Background: Self-assembled mannan nanogels are designed to provide a therapeutic or vaccine delivery platform based on the bioactive properties of mannan to target mannose receptor expressed on the surface of antigen-presenting cells, combined with the performance of nanogels as carriers of biologically active agents. Methods: Proteins in the corona around mannan nanogel formed in human plasma were identified by mass spectrometry after size exclusion chromatography or centrifugation followed by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Structural changes and time dependent binding of human apolipoprotein A-I (apoA-I) and human serum albumin (HSA) to mannan nanogel were studied using intrinsic tryptophan fluorescence and circular dichroism spectroscopy. The mannan nanogel effect on blood coagulation and fibrillation of Alzheimer's disease-associated amyloid beta peptide and hemodialysis-associated amyloidosis beta 2 microglobulin was evaluated using thrombin generation assay or thioflavin T fluorescence assay, respectively. Results: The protein corona around mannan nanogel is formed through a slow process, is quite specific comprising apolipoproteins B-100, A-I and E and HSA, evolves over time, and the equilibrium is reached after hours to days. Structural changes and time dependent binding of apoA-I and HSA to mannan nanogel are minor. The mannan nanogel does not affect blood coagulation and retards the fibril formation. Conclusions: Mannan nanogel has a high biosafety and biocompatibility, which is mandatory for nanomaterials to be used in biomedical applications. General Significance: Our research provides a molecular approach to evaluate the safety aspects of nanomaterials, which is of general concern in society and science. (C) 2012 Elsevier B.V. All rights reserved.


  • Clinical Chemistry, Malmö
  • Biochemistry and Structural Biology
  • MultiPark: Multidisciplinary research focused on Parkinson´s disease
  • NanoLund: Center for Nanoscience

Publishing year







Biochimica et Biophysica Acta. General Subjects





Document type

Journal article




  • Medicinal Chemistry


  • Mannan nanogel
  • Protein corona
  • Coagulation
  • Fibrillation
  • Biosafety
  • Biocompatibility



Research group

  • Clinical Chemistry, Malmö


  • ISSN: 0304-4165